Expression of MyoD, myogenin, myostatin and Hsp70 transcripts in chicken embryos submitted to mild cold or heat
نویسندگان
چکیده
1. The expression of the MyoD, myogenin, myostatin and Hsp70 genes was estimated in chicken embryos submitted to mild cold (3670.51C) or heat (4470.51C) for 1 h. 2. Marked decreases in MyoD, myogenin and myostatin transcript levels were observed in embryos exposed to high temperature, contrasting to the higher expression of the Hsp70 mRNA detected in heat-stressed embryos. 3. The exposure of chicken embryos to low temperature significantly affected only the abundance of myogenin mRNA. 4. These findings suggest that myogenic proliferation and differentiation events are compromised by variations in environmental temperature during avian embryogenesis. r 2003 Elsevier Science Ltd. All rights reserved.
منابع مشابه
Abundance of MyoD and myostatin transcripts in chicken embryos submitted to distinct incubation temperatures and timing exposures.
563 The formation of skeletal muscle tissue is directly modulated by intricate expressing the distinct signalling molecules orchestrated by the myogenic regulatory factors, such as the myogenic determination factor MyoD, that activates the transcription of muscle-specific genes by binding to consensus DNA sites found in the regulatory sequences of these genes, differentiate and fuse the myoblas...
متن کاملExpression of hsp90 Alpha and hsp90 Beta during Xenopus laevis Embryonic Development
Background: Members of the eukaryotic Hsp90 family function as important molecular chaperones in the assembly, folding and activation of cellular signaling in development. Two hsp90 genes, hsp90 alpha and hsp90 beta, have been identified in fish and homeothermic vertebrates but not in poikilothermic vertebrates. In the present study, the expression of hsp90 alpha and hsp90 beta genes in Xenopus...
متن کاملThe Expression of Myogenin and Myostatin Genes in Baluchi Sheep
Myogenin gene (MYoG) affects the synthesis of muscle myofibrillar growth and increase of meat production. The myostatin (MSTN) gene is identified as a specific negative regulator of skeletal muscle growth. Reduction of the expression level of MSTN throughmutation in the sequence of this gene leads to an increase of myogenesis and regeneration of muscle cells during the postnatal growing period ...
متن کاملThe Combined Effect of High-Intensity Interval Training and Metformin on Gene Expression of Myogenin and Myostatin in Skeletal Muscle of Type 2 Diabetic Mice
Background: Myogenin (MyoG) and Myostatin (Mstn) play role in muscle growth and wasting, respectively. The present study aimed to investigate the combined effect of High-intensity Interval Training (HIIT) and Metformin drug (Metf) on gene expression of MyoG and Mstn in skeletal muscle of type 2 diabetic mice. Methods: 25 mice (C57BL/6) were assigned to two groups, including 1) Control © (n=5),...
متن کاملUrsolic Acid Improve Skeletal Muscle Hypertrophy by Increasing of PAX7, Myod and Myogenin Expression and Satellite Cells Proliferation in Native Broiler Chickens
Ursolic acid (UA) is known as a naturally occurring triterpene pentacyclic compound in some medicinal herbs including savory that affects the skeletal muscle. In the current study, the effect of UA was evaluated on C2C12 cells and satellite cells (SCs) isolated from native broiler chicks. First in the in vitro experiment, the C2C12 cell line obtained from the Stem Cell Technology Research Cente...
متن کامل